Detection limits of Legionella pneumophila in environmental samples after co-culture with Acanthamoeba polyphaga
نویسندگان
چکیده
منابع مشابه
molecular detection of legionella pneumophila within culture-negative samples
introduction: l. pneumophila is the most important cause of legionaires, disease, which is currently reported either as a nosocomial or community acquired infection. rapid and reliable diagnosis of legionella has hampered the epidemiological studies and disease control activities. in spite of good sensitivity and specificity, the culture method encountered serious shortcomings. polymerase chain...
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Free-living amoebae in water are hosts to many bacterial species living in such an environment. Such an association enables bacteria to select virulence factors and survive in adverse conditions. Waterborne mycobacteria (WBM) are important sources of community- and hospital-acquired outbreaks of nontuberculosis mycobacterial infections. However, the interactions between WBM and free-living amoe...
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Over a Period of three months (summer), 108 samples of water collected from four hospitals in Te-hran. They were taken from numerous potable and non-potable water, including main supply cis-terns, calorifiers, hot and cold tap water, shower, cooling tower and drain of air condition system. Culturing of concentrated and non-concentrated water samples on Buffered Charcoal Yeast Extract (BCYE) Aga...
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Legionella pneumophila is a ubiquitous, pathogenic, Gram-negative bacterium responsible for legionellosis. Like many other amoeba-resistant microorganisms, L. pneumophila resists host clearance and multiplies inside the cell. Through its Dot/Icm type IV secretion system, the bacterium injects more than three hundred effectors that modulate host cell physiology in order to promote its own intrac...
متن کاملCharacteristics of environmental isolates of Legionella pneumophila.
Thirty-eight cultures of Legionella pneumophila isolated from surface waters were characterized by their morphological, tinctorial, biochemical, and serological properties and by their ability to produce disease in guinea pigs. Their susceptibility to antimicrobial agents also was tested. When they were compared with clinical isolates, no important differences were found between cultures from ...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2013
ISSN: 1471-2180
DOI: 10.1186/1471-2180-13-49